International Journal of Fisheries and Aquatic Studies
Volume 1, Issue 1 2013
Tissue and Substrate Specific Esterase Isozyme Banding Pattern in Macrobrachium rosenbergii
Author(s): Md. Abdur Rashid *, Mohammad Kamruzzaman
Abstract: Tissue and substrate specificity of esterase isozymes was studied from five different tissues of Macrobrachium rosenbergii on polyacrylamide gels (7.5 %) stained with α or β or both α and β naphthyl acetates together. Altogether, five esterase bands (Est-1, Est-2, Est-3, Est-4 and Est-5) were observed that showed both tissue and substrate specific expression. Maximum, four esterase bands were found in eye (Est-1, Est-3, Est-4 and Est-5) and tail muscle (Est-1, Est-2, Est-4 and Est-5) while other tissues showed three bands stained with both α and β naphthyl acetates together. Est-5 was highly expressed (100%) in all the tissues as well as in all three substrates (α or β or α-β naphthyl acetates together). Most of the bands were found to be α-β specific (68 %) while few bands were α (28 %) or β (36%) specific. Both Est-1 and Est-4 were completely absent in α as well as in β stained gels.
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