International Journal of Fisheries and Aquatic Studies
2023, Vol. 11, Issue 2, Part B
Efficacy of Aloe vera variety Barbadensis on bacterial isolates from cultured freshwater fish in Kenya
Author(s): FJ Charo, PG Mbuthia, LC Bebora and JM Nguta
Abstract: Aloe vera variety
barbadensis is a potential alternative antimicrobial plant extract that has been widely used in humans. The objective of this study was to investigate the susceptibility of selected bacterial isolates from cultured freshwater fish to different concentrations of
Aloe vera variety
barbadensis crude extract. Using Minimum inhibitory concentration method by Broth dilution technique, the extract was diluted in distilled water to concentrations of 250 mg/ml, 125mg/ml, 62.5mg/ml, 31.25mg.ml, 15.63mg/ml,7.85 mg/ml, 3.93 mg/ml and 1.96 mg/ml. Eighteen (18) bacterial isolates namely
Enterococcus faecalis NT2I3,
Lysinibacillus fusiformis MT8G4,
Bacillus cereus NC5K2,
Bacillus cereus (MT7G3),
Micrococcus luteus (MC3K2),
Kurthia gibsoni (NT5G5),
Bacillus thuringiensis NT4G4,
Aeromonas hydrophila NT5K2,
Aeromonas hydrophila MT7G2,
Pseudomonas mendocina NT5G2,
Pseudomonas putida MT7I5,
Enterobacter cloaca MT6G2,
Aeromonas veronii NT4G1,
Citrobacter braakii NC3S2,
Citrobacter freundii NC3G3,
Escherichia coli NC2G1(ii),
Escherichia coli MT2G1 and
Hafnia alvei MC3K1were each suspended in physiological saline to a turbidity matching 0.5 Mc Farland opacity tube. Then for each extract concentration, 1 ml was mixed with 0.1 ml of respective bacterial suspension and incubated at 37 °C for 24 hours. Only 1 isolate (
Kurthia gibsonii) was susceptible at a MIC of 62.5 mg/ml of
Aloe vera. Seven isolates (
Bacillus cereus MT7G3,
Micrococcus luteus,
Aeromonas hydrophila NT5K2,
Aeromonas hydrophila MT7G3,
Aeromonas veronii,
Citrobacter braakii and
Escherichia coli NC2G1 (ii)) were susceptible at a MIC of 125 mg/ml of
Aloe vera. Five isolates (
Bacillus cereus NC3K2,
Bacillus thuringiensis,
Pseudomonas putida,
Enterobacter cloaca and
Enterococcus feacalis) were susceptible at a MIC of 250mg/ml of
Aloe vera. Resistance to the highest concentration of
Aloe vera was observed in five isolates namely Lysinibacillus fusiformis, Pseudomonas mendocina, Citrobacter freundii, Hafnia alvei and Escherichia coli MT2G1.
Aloe vera variety
barbadensis extract was shown to be efficacious against most Gram positive and Gram negative bacteria isolated from cultured fish at a concentration between 125 mg/ml to 250 mg/ml.
DOI: 10.22271/fish.2023.v11.i2b.2797Pages: 110-113 | 638 Views 447 DownloadsDownload Full Article: Click Here
How to cite this article:
FJ Charo, PG Mbuthia, LC Bebora, JM Nguta.
Efficacy of Aloe vera variety Barbadensis on bacterial isolates from cultured freshwater fish in Kenya. Int J Fish Aquat Stud 2023;11(2):110-113. DOI:
https://doi.org/10.22271/fish.2023.v11.i2b.2797
